| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NGB (1-151) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:10-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NGB |
| Gene ID: |
58157 |
| Uniprot ID: |
NGB_HUMAN |
| Immunogen Region: |
1-151 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1-151 of human NGB (NP_067080.1). |
| Immunogen Sequence: |
MERPEPELIRQSWRAVSRSP LEHGTVLFARLFALEPDLLP LFQYNCRQFSSPEDCLSSPE FLDHIRKVMLVIDAAVTNVE DLSSLEEYLASLGRKHRAVG VKLSSFSTVGESLLYMLEKC LGPAFTPATRAAWSQLYGAV VQAMSRGWDGE |
| Tissue Specificity | Predominantly expressed in brain, the strongest expression is seen in the frontal lobe, the subthalamic nucleus and the thalamus. |
| Post Translational Modifications | A redox disulfide bond regulates the heme pocket coordination and the rate of nitrite reduction to NO. Phosphorylated in vitro by ERK1, ERK2 and PKA, and in vivo during hypoxia. Phosphorylation increases nitrite reductase activity. |
| Function | Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis. |
| Protein Name | Neuroglobin |
| Database Links | Reactome: R-HSA-8981607 |
| Cellular Localisation | PerikaryonCytoplasmMitochondrion |
| Alternative Antibody Names | Anti-Neuroglobin antibodyAnti-NGB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
