Anti-NFYB antibody (1-50) (STJ94456)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human NFYB. AA range:1-50
General Information
| Short Description | Rabbit polyclonal anti-Nuclear transcription factor Y subunit beta (1-50) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NFYB |
| Gene ID | 4801 |
| Uniprot ID | NFYB_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NFYB. AA range:1-50 |
| Immunogen Region | 1-50 |
| Specificity | NF-YB Polyclonal Antibody detects endogenous levels of NF-YB protein. |
Additional Info
| Post Translational Modifications | Monoubiquitination at Lys-140 plays an important role in transcriptional activation by allowing the deposition of histone H3 methylations as well as histone H2B monoubiquitination at 'Lys-121'. |
| Function | Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. |
| Protein Name | Nuclear Transcription Factor Y Subunit BetaCaat Box Dna-Binding Protein Subunit BNuclear Transcription Factor Y Subunit BNf-Yb |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-2426168Reactome: R-HSA-380994Reactome: R-HSA-381183Reactome: R-HSA-9614657 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Nuclear Transcription Factor Y Subunit Beta antibodyAnti-Caat Box Dna-Binding Protein Subunit B antibodyAnti-Nuclear Transcription Factor Y Subunit B antibodyAnti-Nf-Yb antibodyAnti-NFYB antibodyAnti-HAP3 antibody |
Information sourced from Uniprot.org