| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NFS1 (208-457) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NFS1 |
| Gene ID: |
9054 |
| Uniprot ID: |
NFS1_HUMAN |
| Immunogen Region: |
208-457 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 208-457 of human NFS1 (NP_066923.3). |
| Immunogen Sequence: |
EIGVKQPIAEIGRICSSRKV YFHTDAAQAVGKIPLDVNDM KIDLMSISGHKIYGPKGVGA IYIRRRPRVRVEALQSGGGQ ERGMRSGTVPTPLVVGLGAA CEVAQQEMEYDHKRISKLSE RLIQNIMKSLPDVVMNGDPK HHYPGCINLSFAYVEGESLL MALKDVALSSGSACTSASLE PSYVLRAIGTDEDLAHSSIR FGIGRFTTEEEVDYTVEKCI QHVKRLREMSPLWEMVQDG |
| Tissue Specificity | Predominantly expressed in heart and skeletal muscle. Also found in brain, liver and pancreas. |
| Post Translational Modifications | N-gluconoylated. Cysteine persulfide intermediate is reduced by thiol-containing molecules like glutathione and L-cysteine. Persulfide reduction is a rate-limiting step of cysteine desulfurase catalytic cycle. |
| Function | Isoform Mitochondrial: Cysteine desulfurase, of the core iron-sulfur cluster (ISC) assembly complex, that catalyzes the desulfuration of L-cysteine to L-alanine, as component of the cysteine desulfurase complex, leading to the formation of a cysteine persulfide intermediate at the active site cysteine residue and participates in the 2Fe-2S clusters assembly on the scaffolding protein ISCU. The persulfide is then transferred on the flexible Cys loop from the catalytic site of NFS1 to the surface of NFS1. After the NFS1-linked persulfide sulfur is transferred to one of the conserved Cys residues of the scaffold, a reaction assisted by FXN. The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a 2Fe-2S cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the 2Fe-2S cluster assembly. Finally, the 2Fe-2S cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5. Isoform Cytoplasmic: May catalyze the desulfuration of L-cysteine to L-alanine as component of the cysteine desulfurase complex (NFS1:LYRM4), leading to the formation of a cysteine persulfide intermediate. Acts as a sulfur donor for MOCS3 by transferring the sulfur of the cysteine persulfide intermediate on MOCS3. |
| Protein Name | Cysteine Desulfurase |
| Database Links | Reactome: R-HSA-1362409 Q9Y697-1Reactome: R-HSA-947581 Q9Y697-2 |
| Cellular Localisation | Isoform Mitochondrial: MitochondrionIsoform Cytoplasmic: CytoplasmNucleusCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosome |
| Alternative Antibody Names | Anti-Cysteine Desulfurase antibodyAnti-NFS1 antibodyAnti-NIFS antibodyAnti-HUSSY-08 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
