Western blot analysis of lysates from A-549 cells, using NFIX Rabbit pAb (STJ113639) at 1:600 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-NFIX antibody (180-260) (STJ113639)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ113639
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General Information

Short DescriptionRabbit polyclonal NFIX (180-260) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolNFIX
Gene ID4784
Uniprot IDNFIX_HUMAN
Immunogen Region180-260
Immunogen SequenceAYFVHTPESGQSDSSNQQGD ADIKPLPNGHLSFQDCFVTS GVWNVTELVRVSQTPVATAS GPNFSLADLESPSYYNINQV T
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 180-260 of human NFIX (NP_002492.2).

Additional Info

Tissue Specificity Widely expressed.
Function Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Protein Name Nuclear Factor 1 X-Type
Nf1-X
Nuclear Factor 1/X
Ccaat-Box-Binding Transcription Factor
Ctf
Nuclear Factor I/X
Nf-I/X
Nfi-X
Tggca-Binding Protein
Database Links Reactome: R-HSA-73980
Reactome: R-HSA-749476
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Factor 1 X-Type antibody
Anti-Nf1-X antibody
Anti-Nuclear Factor 1/X antibody
Anti-Ccaat-Box-Binding Transcription Factor antibody
Anti-Ctf antibody
Anti-Nuclear Factor I/X antibody
Anti-Nf-I/X antibody
Anti-Nfi-X antibody
Anti-Tggca-Binding Protein antibody
Anti-NFIX antibody

Information sourced from Uniprot.org

Citations

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