• Western blot analysis of extracts of various cell lines, using NFIX antibody (STJ113639) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Western blot analysis of lysates from A-549 cells, using NFIX Rabbit polyclonal antibody (STJ113639) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-NFIX antibody (180-260) (STJ113639)

SKU:
STJ113639

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFIX
Gene ID: 4784
Uniprot ID: NFIX_HUMAN
Immunogen Region: 180-260
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 180-260 of human NFIX (NP_002492.2).
Immunogen Sequence: AYFVHTPESGQSDSSNQQGD ADIKPLPNGHLSFQDCFVTS GVWNVTELVRVSQTPVATAS GPNFSLADLESPSYYNINQV T
Tissue Specificity Widely expressed.
Function Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Protein Name Nuclear Factor 1 X-Type
Nf1-X
Nuclear Factor 1/X
Ccaat-Box-Binding Transcription Factor
Ctf
Nuclear Factor I/X
Nf-I/X
Nfi-X
Tggca-Binding Protein
Database Links Reactome: R-HSA-73980
Reactome: R-HSA-749476
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Factor 1 X-Type antibody
Anti-Nf1-X antibody
Anti-Nuclear Factor 1/X antibody
Anti-Ccaat-Box-Binding Transcription Factor antibody
Anti-Ctf antibody
Anti-Nuclear Factor I/X antibody
Anti-Nf-I/X antibody
Anti-Nfi-X antibody
Anti-Tggca-Binding Protein antibody
Anti-NFIX antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance