Anti-NFIL3 antibody (133-462) (STJ115964)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NFIL3 (133-462) antibody for use in WB, IF, ICC and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NFIL3 |
| Gene ID | 4783 |
| Uniprot ID | NFIL3_HUMAN |
| Immunogen Region | 133-462 |
| Immunogen Sequence | AYAQEIQKLSNSTAVYFQDY QTSKSNVSSFVDEHEPSMVS SSCISVIKHSPQSSLSDVSE VSSVEHTQESSVQGSCRSPE NKFQIIKQEPMELESYTREP RDDRGSYTASIYQNYMGNSF SGYSHSPPLLQVNRSSSNSP RTSETDDGVVGKSSDGEDEQ QVPKGPIHSPVELKHVHATV VKVPEVNSSALPHKLRIKAK AMQIKVEAFDNEFEATQKLS SPIDMTSKRHFELEKHSAP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 133-462 of human NFIL3 (NP_005375.2). |
Additional Info
| Tissue Specificity | Expressed in bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle. |
| Function | Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-GATTACTGTAACT-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B-site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death. Represses the transcription of CYP2A5. Positively regulates the expression and activity of CES2 by antagonizing the repressive action of NR1D1 on CES2. Required for the development of natural killer cell precursors. |
| Protein Name | Nuclear Factor Interleukin-3-Regulated ProteinE4 Promoter-Binding Protein 4Interleukin-3 Promoter Transcriptional ActivatorInterleukin-3-Binding Protein 1Transcriptional Activator Nf-Il3a |
| Database Links | Reactome: R-HSA-400253 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Nuclear Factor Interleukin-3-Regulated Protein antibodyAnti-E4 Promoter-Binding Protein 4 antibodyAnti-Interleukin-3 Promoter Transcriptional Activator antibodyAnti-Interleukin-3-Binding Protein 1 antibodyAnti-Transcriptional Activator Nf-Il3a antibodyAnti-NFIL3 antibodyAnti-E4BP4 antibodyAnti-IL3BP1 antibody |
Information sourced from Uniprot.org