• Western blot analysis of extracts of various cell lines, using NFIB/ NF1B2 antibody (STJ11103649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using NFIB/ NF1B2 antibody (STJ11103649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NFIB/NF1B2 antibody (321-420) [S9MR] (STJ11103649)

SKU:
STJ11103649

£129.50 - £574.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFIB
Gene ID: 4781
Uniprot ID: NFIB_HUMAN
Immunogen Region: 321-420
Specificity: A synthetic peptide corresponding to a sequence within amino acids 321-420 of human NFIB/NF1B2 (NP_005587.2).
Immunogen Sequence: KPLFSSASPQDSSPRLSTFP QHHHPGIPGVAHSVISTRTP PPPSPLPFPTQAILPPAPSS YFSHPTIRYPPHLNPQDTLK NYVPSYDPSSPQTSQSWYLG
Function Transcriptional activator of GFAP, essential for proper brain development. Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Protein Name Nuclear Factor 1 B-Type
Nf1-B
Nuclear Factor 1/B
Ccaat-Box-Binding Transcription Factor
Ctf
Nuclear Factor I/B
Nf-I/B
Nfi-B
Tggca-Binding Protein
Database Links Reactome: R-HSA-73980
Reactome: R-HSA-749476
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Factor 1 B-Type antibody
Anti-Nf1-B antibody
Anti-Nuclear Factor 1/B antibody
Anti-Ccaat-Box-Binding Transcription Factor antibody
Anti-Ctf antibody
Anti-Nuclear Factor I/B antibody
Anti-Nf-I/B antibody
Anti-Nfi-B antibody
Anti-Tggca-Binding Protein antibody
Anti-NFIB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance