• Western blot analysis of extracts of mouse brain, using NFIA antibody (STJ24756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using NFIA rabbit polyclonal antibody (STJ24756) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using NFIA rabbit polyclonal antibody (STJ24756) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using NFIA rabbit polyclonal antibody (STJ24756) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using NFIA rabbit polyclonal antibody (STJ24756) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using NFIA rabbit polyclonal antibody (STJ24756) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using NFIA rabbit polyclonal antibody (STJ24756) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NFIA antibody (180-330) (STJ24756)

SKU:
STJ24756

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:200-1:2000
IHC-P:1:50-1:200
IF:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFIA
Gene ID: 4774
Uniprot ID: NFIA_HUMAN
Immunogen Region: 180-330
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 180-330 of human NFIA (NP_005586.1).
Immunogen Sequence: AYFVHAADSSQSESPSQPSD ADIKDQPENGHLGFQDSFVT SGVFSVTELVRVSQTPIAAG TGPNFSLSDLESSSYYSMSP GAMRRSLPSTSSTSSTKRLK SVEDEMDSPGEEPFYTGQGR SPGSGSQSSGWHEVEPGMPS PTTLKKSEKSG
Function Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Protein Name Nuclear Factor 1 A-Type
Nf1-A
Nuclear Factor 1/A
Ccaat-Box-Binding Transcription Factor
Ctf
Nuclear Factor I/A
Nf-I/A
Nfi-A
Tggca-Binding Protein
Database Links Reactome: R-HSA-73980
Reactome: R-HSA-749476
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Factor 1 A-Type antibody
Anti-Nf1-A antibody
Anti-Nuclear Factor 1/A antibody
Anti-Ccaat-Box-Binding Transcription Factor antibody
Anti-Ctf antibody
Anti-Nuclear Factor I/A antibody
Anti-Nf-I/A antibody
Anti-Nfi-A antibody
Anti-Tggca-Binding Protein antibody
Anti-NFIA antibody
Anti-KIAA1439 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance