Anti-NFATC4/NFAT3 antibody (Internal) (STJ71974)

SKU:
STJ71974-100

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Host: Goat
Applications: Pep-ELISA
Reactivity: Human/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-NFATC4/NFAT3 (Internal) is suitable for use in ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NFATC4
Gene ID: 4776
Uniprot ID: NFAC4_HUMAN
Immunogen Region: Internal
Accession Number: NP_001129494.1; NP_004545.2
Specificity: This antibody is expected to recognize both isoforms (NP_001129494.1; NP_004545.2).
Immunogen Sequence: EATVNRLQSNEVTLT
Post Translational Modifications Phosphorylated by NFATC-kinases.dephosphorylated by calcineurin/PPP3CA. Phosphorylated on Ser-168 and Ser-170 by MTOR, IRAK1, MAPK7/ERK5 and MAPK14/p38, on Ser-213 and Ser-217 by MAPK8/JNK1 and MAPK9/JNK2, and on Ser-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B. Phosphorylation by GSK3B markedly increases NFATC4 ubiquitination. Phosphorylation at Ser-168 and Ser-170 is stimulated by UV irradiation. Phosphorylation determines subcellular location: the hyperphosphorylated protein is cytosolic, while the dephosphorylated form is targeted to the nucleus. Ubiquitinated, leading to degradation by the proteasome. Ubiquitination may be stimulated by GSK3B-dependent phosphorylation. Polyubiquitin linkage mainly occurs through 'Lys-48'.
Function Ca(2+)-regulated transcription factor that is involved in several processes, including the development and function of the immune, cardiovascular, musculoskeletal, and nervous systems. Involved in T-cell activation, stimulating the transcription of cytokine genes, including that of IL2 and IL4. Along with NFATC3, involved in embryonic heart development. Involved in mitochondrial energy metabolism required for cardiac morphogenesis and function. Transactivates many genes involved in the cardiovascular system, including AGTR2, NPPB/BNP (in synergy with GATA4), NPPA/ANP/ANF and MYH7/beta-MHC. Involved in the regulation of adult hippocampal neurogenesis. Involved in BDNF-driven pro-survival signaling in hippocampal adult-born neurons. Involved in the formation of long-term spatial memory and long-term potentiation. In cochlear nucleus neurons, may play a role in deafferentation-induced apoptosis during the developmental critical period, when auditory neurons depend on afferent input for survival. Binds to and activates the BACE1/Beta-secretase 1 promoter, hence may regulate the proteolytic processing of the amyloid precursor protein (APP). Plays a role in adipocyte differentiation. May be involved in myoblast differentiation into myotubes. Binds the consensus DNA sequence 5'-GGAAAAT-3' (Probable). In the presence of CREBBP, activates TNF transcription. Binds to PPARG gene promoter and regulates its activity. Binds to PPARG and REG3G gene promoters.
Protein Name Nuclear Factor Of Activated T-Cells - Cytoplasmic 4
Nf-Atc4
Nfatc4
T-Cell Transcription Factor Nfat3
Nf-At3
Cellular Localisation Cytoplasm
Cytosol
Nucleus
When Hyperphosphorylated
Localizes In The Cytosol
When Intracellular Ca(2+) Levels Increase
Dephosphorylation By Calcineurin/Ppp3ca Leads To Translocation Into The Nucleus
Mapk7/Erk5 And Mtor Regulate Nfatc4 Nuclear Export Through Phosphorylation At Ser-168 And Ser-170
Alternative Antibody Names Anti-Nuclear Factor Of Activated T-Cells - Cytoplasmic 4 antibody
Anti-Nf-Atc4 antibody
Anti-Nfatc4 antibody
Anti-T-Cell Transcription Factor Nfat3 antibody
Anti-Nf-At3 antibody
Anti-NFATC4 antibody
Anti-NFAT3 antibody

Information sourced from Uniprot.org

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