Anti-NFAT5 antibody (1355-1455) (STJ119070)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NFAT5 (1355-1455) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NFAT5 |
| Gene ID | 10725 |
| Uniprot ID | NFAT5_HUMAN |
| Immunogen Region | 1355-1455 |
| Immunogen Sequence | NQLQNSPGSSQQTSGMFLFG IQNNCSQLLTSGPATLPDQL MAISQPGQPQNEGQPPVTTL LSQQMPENSPLASSINTNQN IEKIDLLVSLQNQGNNLTGS F |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1355-1455 of human NFAT5 (NP_619728.2). |
Additional Info
| Tissue Specificity | Widely expressed, with highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes. |
| Post Translational Modifications | Phosphorylated. Phosphorylated at Thr-135 by CDK5 in response to osmotic stress.this phosphorylation mediates its rapid nuclear localization. Poly-ADP-ribosylated by PARP1 in response to DNA damage, promoting recruitment to sites of R-loop-associated DNA damage. |
| Function | Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-ACTAGTGGAAACATATAATCCAATGGTGACCGCT-3'. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes.optimal transactivation of these genes requires the presence of DDX5/DDX17. Also involved in the DNA damage response by preventing formation of R-loops.R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA. |
| Protein Name | Nuclear Factor Of Activated T-Cells 5Nf-At5T-Cell Transcription Factor Nfat5Tonicity-Responsive Enhancer-Binding ProteinTone-Binding ProteinTonebp |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmChromosomeNuclear Distribution Increases Under Hypertonic ConditionsRecruited To Sites Of R-Loop-Associated Dna Damage Following Poly-Adp-Ribosylation By Parp1 |
| Alternative Antibody Names | Anti-Nuclear Factor Of Activated T-Cells 5 antibodyAnti-Nf-At5 antibodyAnti-T-Cell Transcription Factor Nfat5 antibodyAnti-Tonicity-Responsive Enhancer-Binding Protein antibodyAnti-Tone-Binding Protein antibodyAnti-Tonebp antibodyAnti-NFAT5 antibodyAnti-KIAA0827 antibodyAnti-TONEBP antibody |
Information sourced from Uniprot.org