| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NFAT5 (1355-1455) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NFAT5 |
| Gene ID: |
10725 |
| Uniprot ID: |
NFAT5_HUMAN |
| Immunogen Region: |
1355-1455 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1355-1455 of human NFAT5 (NP_619728.2). |
| Immunogen Sequence: |
NQLQNSPGSSQQTSGMFLFG IQNNCSQLLTSGPATLPDQL MAISQPGQPQNEGQPPVTTL LSQQMPENSPLASSINTNQN IEKIDLLVSLQNQGNNLTGS F |
| Tissue Specificity | Widely expressed, with highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes. |
| Post Translational Modifications | Phosphorylated. Phosphorylated at Thr-135 by CDK5 in response to osmotic stress.this phosphorylation mediates its rapid nuclear localization. Poly-ADP-ribosylated by PARP1 in response to DNA damage, promoting recruitment to sites of R-loop-associated DNA damage. |
| Function | Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-ACTAGTGGAAACATATAATCCAATGGTGACCGCT-3'. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes.optimal transactivation of these genes requires the presence of DDX5/DDX17. Also involved in the DNA damage response by preventing formation of R-loops.R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA. |
| Protein Name | Nuclear Factor Of Activated T-Cells 5Nf-At5T-Cell Transcription Factor Nfat5Tonicity-Responsive Enhancer-Binding ProteinTone-Binding ProteinTonebp |
| Cellular Localisation | NucleusCytoplasmChromosomeNuclear Distribution Increases Under Hypertonic ConditionsRecruited To Sites Of R-Loop-Associated Dna Damage Following Poly-Adp-Ribosylation By Parp1 |
| Alternative Antibody Names | Anti-Nuclear Factor Of Activated T-Cells 5 antibodyAnti-Nf-At5 antibodyAnti-T-Cell Transcription Factor Nfat5 antibodyAnti-Tonicity-Responsive Enhancer-Binding Protein antibodyAnti-Tone-Binding Protein antibodyAnti-Tonebp antibodyAnti-NFAT5 antibodyAnti-KIAA0827 antibodyAnti-TONEBP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
