| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Merlin (347-595) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NF2 |
| Gene ID: |
4771 |
| Uniprot ID: |
MERL_HUMAN |
| Immunogen Region: |
347-595 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 347-595 of human Merlin. (NP_000259.1). |
| Immunogen Sequence: |
EEAERTRDELERRLLQMKEE ATMANEALMRSEETADLLAE KAQITEEEAKLLAQKAAEAE QEMQRIKATAIRTEEEKRLM EQKVLEAEVLALKMAEESER RAKEADQLKQDLQEAREAER RAKQKLLEIATKPTYPPMNP IPAPLPPDIPSFNLIGDSLS FDFKDTDMKRLSMEIEKEKV EYMEKSKHLQEQLNELKTEI EALKLKERETALDILHNENS DRGGSSKHNTIKKLTLQSA |
| Tissue Specificity | Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels. |
| Post Translational Modifications | Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail. The dephosphorylation of Ser-518 favors the interaction with NOP53. Ubiquitinated by the CUL4A-RBX1-DDB1-DCAF1/VprBP E3 ubiquitin-protein ligase complex for ubiquitination and subsequent proteasome-dependent degradation. |
| Function | Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. |
| Protein Name | MerlinMoesin-Ezrin-Radixin-Like ProteinNeurofibromin-2SchwannomerlinSchwannomin |
| Database Links | Reactome: R-HSA-2029482Reactome: R-HSA-5627123 |
| Cellular Localisation | Isoform 1: Cell ProjectionFilopodium MembranePeripheral Membrane ProteinCytoplasmic SideCell ProjectionRuffle MembraneNucleusIn A Fibroblastic Cell LineIsoform 1 Is Found Homogeneously Distributed Over The Entire CellWith A Particularly Strong Staining In Ruffling Membranes And FilopodiaColocalizes With Mpp1 In Non-Myelin-Forming Schwann CellsBinds With Dcaf1 In The NucleusThe Intramolecular Association Of The Ferm Domain With The C-Terminal Tail Promotes Nuclear AccumulationThe Unphosphorylated Form Accumulates Predominantly In The Nucleus While The Phosphorylated Form Is Largely Confined To The Non-Nuclear FractionsIsoform 7: CytoplasmPerinuclear RegionCytoplasmic GranuleObserved In Cytoplasmic Granules Concentrated In A Perinuclear LocationIsoform 7 Is Absent From Ruffling Membranes And FilopodiaIsoform 9: CytoplasmIsoform 9 Is Absent From Ruffling Membranes And FilopodiaIsoform 10: NucleusCytoplasmCytoskeletonIsoform 10 Is Found Homogeneously Distributed Over The Entire Cell |
| Alternative Antibody Names | Anti-Merlin antibodyAnti-Moesin-Ezrin-Radixin-Like Protein antibodyAnti-Neurofibromin-2 antibodyAnti-Schwannomerlin antibodyAnti-Schwannomin antibodyAnti-NF2 antibodyAnti-SCH antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunoprecipitation analysis of 200 Mu g extracts of A-549 cells using 3 Mu g [KD Validated] NF2 Rabbit polyclonal antibody (STJ115585). Western blot was performed from the immunoprecipitate using [KO Validated] NF2 Rabbit polyclonal antibody (STJ115585) at a dilition of 1:1000.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/94601/376322/STJ115585_1__30832.1713143345.jpg?c=1 "Immunoprecipitation analysis of 200 Mu g extracts of A-549 cells using 3 Mu g [KD Validated] NF2 Rabbit polyclonal antibody (STJ115585). Western blot was performed from the immunoprecipitate using [KO Validated] NF2 Rabbit polyclonal antibody (STJ115585) at a dilition of 1:1000.")
![Immunoprecipitation analysis of 200 Mu g extracts of SKOV3 cells using 3 Mu g [KD Validated] NF2 Rabbit polyclonal antibody (STJ114897). Western blot was performed from the immunoprecipitate using NF2 antibody (STJ114897) at a dilution of 1:1000.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/93966/375271/STJ114897_1__38068.1713141997.jpg?c=1 "Immunoprecipitation analysis of 200 Mu g extracts of SKOV3 cells using 3 Mu g [KD Validated] NF2 Rabbit polyclonal antibody (STJ114897). Western blot was performed from the immunoprecipitate using NF2 antibody (STJ114897) at a dilution of 1:1000.")