Anti-Neurofilament light polypeptide antibody [1D44] (STJA0040824)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenThe antibody has been made against a proprietary recombinant construct containing amino acids of human NF-L expressed in and purified from E Coli.
General Information
| Short Description | Mouse monoclonal anti-Neurofilament light polypeptide for use in ELISA, IF, ICC, IHC and WB in Bovine, Human, Mouse, Pig and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/IF/ICC/IHC/WB |
| Host | Mouse |
| Reactivity | Bovine/Human/Mouse/Pig/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 1D44 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | IgG |
| Dilution Range | WB 1:1000-1:2000ICC 0.73611111111111116 |
| Formulation | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Storage Instruction | Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C After reconstitution of lyophilized anti |
Target Information
| Gene Symbol | NEFL |
| Gene ID | 4747 |
| Uniprot ID | NFL_HUMAN |
| Immunogen | The antibody has been made against a proprietary recombinant construct containing amino acids of human NF-L expressed in and purified from E Coli. |
| Immunogen Sequence | Human |
| Specificity | Species cross-reactivity includes human, rat, mouse, cow, and pig. These are antibodies which recognize epitopes in a small segment of the neurofilament NF-L subunit which are normally not accessible to antibodies but which became available on degene |
Additional Info
| Post Translational Modifications | O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1. |
| Function | Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. |
| Protein Name | Neurofilament Light PolypeptideNf-L68 Kda Neurofilament ProteinNeurofilament Triplet L Protein |
| Database Links | Reactome: R-HSA-438066Reactome: R-HSA-442982Reactome: R-HSA-5673001Reactome: R-HSA-9609736Reactome: R-HSA-9617324Reactome: R-HSA-9620244 |
| Cellular Localisation | Cell ProjectionAxonCytoplasmCytoskeleton |
| Alternative Antibody Names | Anti-Neurofilament Light Polypeptide antibodyAnti-Nf-L antibodyAnti-68 Kda Neurofilament Protein antibodyAnti-Neurofilament Triplet L Protein antibodyAnti-NEFL antibodyAnti-NF68 antibodyAnti-NFL antibody |
Information sourced from Uniprot.org