Western blot analysis of various lysates using NeuN Mouse mAb (STJ11107255) at 1:5000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-NeuN antibody [SMM] (STJ11107255)

SKU:
STJ11107255
£146.50 - £642.50
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Host: Mouse
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : SMM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:2500-1:5000
IHC-P:1:200-1:800
IF/ICC:1:200-1:800
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Gene Symbol: RBFOX3
Gene ID: 146713
Uniprot ID: RFOX3_HUMAN
Specificity: Recombinant protein of human NeuN.
Function Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).
Protein Name Rna Binding Protein Fox-1 Homolog 3
Fox-1 Homolog C
Neuronal Nuclei Antigen
Neun Antigen
Database Links Reactome: R-HSA-9768919
Cellular Localisation Nucleus
Cytoplasm
Largely Restricted To Neuronal Nuclei
However
Significant Cytoplasmic Localization In Neurons From Brains From Hiv-Infected Individuals With Cognitive Impairment
Alternative Antibody Names Anti-Rna Binding Protein Fox-1 Homolog 3 antibody
Anti-Fox-1 Homolog C antibody
Anti-Neuronal Nuclei Antigen antibody
Anti-Neun Antigen antibody
Anti-RBFOX3 antibody

Information sourced from Uniprot.org

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