| Host: | Chicken |
| Applications: | ICC/WB |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Chicken polyclonal anti-Nestin (315-630) for use in ICC and WB in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgY |
| Formulation: | Lyophilized IgY preparation, with sodium azide. |
| Purification: | IgY preparation |
| Dilution Range: | WB 1:1000-1:5000ICC 1:2000-1:5000 |
| Storage Instruction: | Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at-20°C for long-term s |
| Gene Symbol: | NES |
| Gene ID: | 10763 |
| Uniprot ID: | NEST_HUMAN |
| Immunogen Region: | 315-630 |
| Specificity: | Reacts with human and rat. Other species not tested. |
| Immunogen: | Part of recombinant human protein (amino acids 315-630). |
| Immunogen Sequence: | Human |
| Tissue Specificity | CNS stem cells. |
| Post Translational Modifications | Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. |
| Function | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. |
| Protein Name | Nestin |
| Database Links | |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Nestin antibodyAnti-NES antibodyAnti-Nbla00170 antibody |
Information sourced from Uniprot.org