• Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 kDa nestin doublet.
  • Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (STJA0003696, red, 1:500) and anti-MAP2 antibody (STJA0003676, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
  • Immunostaining of cultured neonatal rat neurons and glia stained with anti-nestin antibody (STJA0003696, red, 1:500) and anti-vimentin antibody (STJA0003823, green, 1:500). The blue is Hoechst staining for nuclear DNA. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.

Anti-NES antibody [4D11] (STJA0003696)

SKU:
STJA0003696-100

£442.50
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Host: Mouse
Applications: WB/IHC/ICC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Nestin is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: 4D11
Conjugation: Unconjugated
Isotype: IgG1
Formulation: 100 ul in PBS + 50% Glycerol and 5 mm Sodium Azide
Purification: This antibody was protein g purified culture from supernatant.
Dilution Range: WB 1:2000
IHC 1:500
ICC 1:500
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NES
Gene ID: 10763
Uniprot ID: NEST_HUMAN
Immunogen: Recombinant human Nestin expressed in and purifeid from E. coli.
Tissue Specificity CNS stem cells.
Post Translational Modifications Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Function Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Protein Name Nestin
Alternative Antibody Names Anti-Nestin antibody
Anti-NES antibody
Anti-Nbla00170 antibody

Information sourced from Uniprot.org

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