Anti-NES antibody (1392-1621) (STJ113858)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Nestin (1392-1621) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NES |
| Gene ID | 10763 |
| Uniprot ID | NEST_HUMAN |
| Immunogen Region | 1392-1621 |
| Immunogen Sequence | QVPQLLLDPAAWDRDGESDG FADEEESGEEGEEDQEEGRE PGAGRWGPGSSVGSLQALSS SQRGEFLESDSVSVSVPWDD SLRGAVAGAPKTALETESQD SAEPSGSEEESDPVSLERED KVPGPLEIPSGMEDAGPGAD IIGVNGQGPNLEGKSQHVNG GVMNGLEQSEEVGQGMPLVS EGDRGSPFQEEEGSALKTSW AGAPVHLGQGQFLKFTQREG DRESWSSGED |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1392-1621 of human NES (NP_006608.1). |
Additional Info
| Tissue Specificity | CNS stem cells. |
| Post Translational Modifications | Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. |
| Function | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. |
| Protein Name | Nestin |
| Database Links | |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Nestin antibodyAnti-NES antibodyAnti-Nbla00170 antibody |
Information sourced from Uniprot.org