• Immunohistochemistry analysis of paraffin-embedded rat kidney using NEK6 Rabbit monoclonal antibody (STJ11101894) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NEK6 Rabbit monoclonal antibody (STJ11101894) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of various cell lines, using NEK6 Rabbit monoclonal antibody (STJ11101894) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-NEK6 antibody (200-300) [S4MR] (STJ11101894)

SKU:
STJ11101894

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-NEK6 (200-300) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEK6
Gene ID: 10783
Uniprot ID: NEK6_HUMAN
Immunogen Region: 200-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human NEK6 (Q9HC98).
Immunogen Sequence: ETTAAHSLVGTPYYMSPERI HENGYNFKSDIWSLGCLLYE MAALQSPFYGDKMNLFSLCQ KIEQCDYPPLPGEHYSEKLR ELVSMCICPDPHQRPDIGYV H
Tissue Specificity Ubiquitous, with highest expression in heart and skeletal muscle.
Post Translational Modifications Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210.
Function Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence. Phosphorylates EML4 at 'Ser-144', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression.
Protein Name Serine/Threonine-Protein Kinase Nek6
Never In Mitosis A-Related Kinase 6
Nima-Related Protein Kinase 6
Protein Kinase Sid6-1512
Database Links Reactome: R-HSA-2980767
Reactome: R-HSA-3301854
Reactome: R-HSA-9648025
Cellular Localisation Cytoplasm
Nucleus
Nucleus Speckle
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle Pole
Colocalizes With Apbb1 At The Nuclear Speckles
Colocalizes With Pin1 In The Nucleus
Colocalizes With Atf4
Cir1
Arhgap33
Ankra2
Cdc42
Nek9
Rad26l
Rbbp6
Rps7
Trip4
Relb And Phf1 In The Centrosome
Localizes To Spindle Microtubules In Metaphase And Anaphase And To The Midbody During Cytokinesis
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Nek6 antibody
Anti-Never In Mitosis A-Related Kinase 6 antibody
Anti-Nima-Related Protein Kinase 6 antibody
Anti-Protein Kinase Sid6-1512 antibody
Anti-NEK6 antibody

Information sourced from Uniprot.org

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