• Western blot analysis of extracts of various cell lines, using TARBP2 rabbit monoclonal antibody (STJ11102424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Confocal imaging of U-2 OS cells using TARBP2 Rabbit monoclonal antibody (STJ11102424, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded rat pancreas using TARBP2 rabbit monoclonal antibody (STJ11102424) at dilution of 1:100 (40x lens).
  • Confocal imaging of NIH/3T3 cells using TARBP2 Rabbit monoclonal antibody (STJ11102424, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded human placenta using TARBP2 rabbit monoclonal antibody (STJ11102424) at dilution of 1:100 (40x lens).
  • Confocal imaging of paraffin-embedded Mouse brain tissue using TARBP2 Rabbit monoclonal antibody (STJ11102424, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry of paraffin-embedded mouse pancreas using TARBP2 rabbit monoclonal antibody (STJ11102424) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of TARBP2 in paraffin-embedded mouse lung tissue using TARBP2 Rabbit monoclonal antibody (STJ11102424) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of NIH-3T3 cells using TARBP2 rabbit monoclonal antibody (STJ11102424) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of TARBP2 in paraffin-embedded mouse kidney tissue using TARBP2 Rabbit monoclonal antibody (STJ11102424) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of U-2 OS cells using TARBP2 rabbit monoclonal antibody (STJ11102424) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of TARBP2 in paraffin-embedded human lung cancer tissue using TARBP2 Rabbit monoclonal antibody (STJ11102424) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of TARBP2 in paraffin-embedded human thyroid cancer tissue using TARBP2 Rabbit monoclonal antibody (STJ11102424) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of TARBP2 in paraffin-embedded human liver tissue using TARBP2 Rabbit monoclonal antibody (STJ11102424) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of TARBP2 in paraffin-embedded human lung tissue using TARBP2 Rabbit monoclonal antibody (STJ11102424) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of TARBP2 in paraffin-embedded rat brain tissue using TARBP2 Rabbit monoclonal antibody (STJ11102424) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using TARBP2 Rabbit monoclonal antibody (STJ11102424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-TARBP2 antibody (200-300) [S4MR] (STJ11102424)

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STJ11102424

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-TRBP (200-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TARBP2
Gene ID: 6895
Uniprot ID: TRBP2_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human TARBP2 (Q15633).
Immunogen Sequence: RFIEIGSGTSKKLAKRNAAA KMLLRVHTVPLDARDGNEVE PDDDHFSIGVGSRLDGLRNR GPGCTWDSLRNSVGEKILSL RSCSLGSLGALGPACCRVLS E
Function Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds in vitro to the PRM1 3'-UTR. Seems to act as a repressor of translation. For some pre-miRNA substrates, may also alter the choice of cleavage site by DICER1. Negatively regulates IRF7-mediated IFN-beta signaling triggered by viral infection by inhibiting the phosphorylation of IRF7 and promoting its 'Lys'-48-linked ubiquitination and degradation. (Microbial infection) Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR. Mediates recruitment of FTSJ3 methyltransferase to HIV-1 RNA, leading to 2'-O-methylation of the viral genome, allowing HIV-1 to escape the innate immune system.
Protein Name Risc-Loading Complex Subunit Tarbp2
Tar Rna-Binding Protein 2
Trans-Activation-Responsive Rna-Binding Protein
Database Links Reactome: R-HSA-203927
Reactome: R-HSA-426486
Cellular Localisation Cytoplasm
Perinuclear Region
Nucleus
Alternative Antibody Names Anti-Risc-Loading Complex Subunit Tarbp2 antibody
Anti-Tar Rna-Binding Protein 2 antibody
Anti-Trans-Activation-Responsive Rna-Binding Protein antibody
Anti-TARBP2 antibody
Anti-TRBP antibody

Information sourced from Uniprot.org

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