• Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.
  • Immunofluorescence of a section of rat frontal cortex showing labeling of Neurofilament-L (STJA0003699, 1:500, red) and labeling of GFAP ( cat. STJA0003659, 1:5000, green). The anti-NFL antibody labels cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells. While the anti-GFAP antibody labels the network of glial cells.
  • Immunostaining of mixed cultured rat neurons stained with anti-NFL antibody (STJA0003699, green, 1:100) and anti-alpha internexin.

Anti-NEFL antibody [DA2] (STJA0003699)

SKU:
STJA0003699-100

£394.50
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Host: Mouse
Applications: WB/IHC/ICC
Reactivity: Bovine/Chicken/Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-NEFL/NF-L is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: DA2
Conjugation: Unconjugated
Isotype: IgG1
Formulation: 100 ul in PBS + 10 mM Sodium Azide.
Purification: This antibody was protein g purified culture from supernatant.
Dilution Range: WB 1:1000
IHC 1:100-1:1000
ICC 1:100-1:1000
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEFL
Gene ID: 4747
Uniprot ID: NFL_HUMAN
Immunogen: Preparation of porcine NF-L.
Post Translational Modifications O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1.
Function Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name Neurofilament Light Polypeptide
Nf-L
68 Kda Neurofilament Protein
Neurofilament Triplet L Protein
Database Links Reactome: R-HSA-438066
Reactome: R-HSA-442982
Reactome: R-HSA-5673001
Reactome: R-HSA-9609736
Reactome: R-HSA-9617324
Reactome: R-HSA-9620244
Cellular Localisation Cell Projection
Axon
Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Neurofilament Light Polypeptide antibody
Anti-Nf-L antibody
Anti-68 Kda Neurofilament Protein antibody
Anti-Neurofilament Triplet L Protein antibody
Anti-NEFL antibody
Anti-NF68 antibody
Anti-NFL antibody

Information sourced from Uniprot.org

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