• Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.
  • Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament H (STJA0003698, 1:25,000, red) in nuclei of neurons and specific labeling of GFAP ( cat. 620-GFAP, 1:5000, green) in astrocytes and other glial cells, and DAPI staining of nuclear DNA.
  • Immunofluorescence of a section of rat cerebellum showing specific labeling of the axons with anti-neurofilament H antibody (STJA0003698, green, 1:25,000). The section is colabeled with anti-calbindin ( cat. STJA0003587, red, 1:1000) labeling the dendrites of Purkinje cells. The blue is DAPI staining nuclear DNA.
  • Immunostaining of mixed cultured rat neurons and glia stained with anti-NFH antibody (STJA0003698, red, 1:25,000) and anti-NFL antibody (STJA0003699, green, 1:100).

Anti-NEFH antibody (STJA0003698)

SKU:
STJA0003698-100

£394.50
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Host: Chicken
Applications: WB/IHC/ICC
Reactivity: All Mammalian/Chicken
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Chicken polyclonal antibody anti-NEFH/NF-H is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgY
Formulation: Total IgY fraction in PBS + 10 mM Sodium Azide.
Purification: This antibody was total igy fraction.
Dilution Range: WB 1:50000
IHC 1:5000-1:25, 000
ICC 1:25, 000
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEFH
Gene ID: 4744
Uniprot ID: NFH_HUMAN
Immunogen: purified bovine NF-H.
Post Translational Modifications There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Function Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name Neurofilament Heavy Polypeptide
Nf-H
200 Kda Neurofilament Protein
Neurofilament Triplet H Protein
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Axon
Alternative Antibody Names Anti-Neurofilament Heavy Polypeptide antibody
Anti-Nf-H antibody
Anti-200 Kda Neurofilament Protein antibody
Anti-Neurofilament Triplet H Protein antibody
Anti-NEFH antibody
Anti-KIAA0845 antibody
Anti-NFH antibody

Information sourced from Uniprot.org

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