STJ72552 (0. 05µg/ml) staining of Rat Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-NEDD8 conjugating enzyme antibody (N-Term) (STJ72552)

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STJ72552-100
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Goat polyclonal antibody anti-NEDD8 conjugating enzyme (N-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx 22kDa band observed in Rat Pancreas lysates (calculated MW of 21.1kDa according to Human NP_542409.1 and 18.6kDa according to Rat NP_001008382.1). Recommended concentration: 0.0
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: UBE2F
Gene ID: 140739
Uniprot ID: UBE2F_HUMAN
Immunogen Region: N-Term
Accession Number: NP_542409.1
Immunogen Sequence: SKLKRDDGLKGSRT
Post Translational Modifications The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.
Function Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. Together with the E3 ubiquitin ligase RNF7/RBX2, specifically neddylates cullin-5 (CUL5). Does not neddylate CUL1, CUL2, CUL3, CUL4A or CUL4B. Mediates neddylation of the CUL9-RBX1 complex. (Microbial infection) Following infection by HIV-1 virus, participates to HIV-1 Vif protein-mediated ubiquitination and degradation of APOBEC3G by mediating neddylation of cullin-5 (CUL5).
Protein Name Nedd8-Conjugating Enzyme Ube2f
Nedd8 Carrier Protein Ube2f
Nedd8 Protein Ligase Ube2f
Nedd8-Conjugating Enzyme 2
Ring-Type E3 Nedd8 Transferase Ube2f
Ubiquitin-Conjugating Enzyme E2 F
Database Links Reactome: R-HSA-8951664
Reactome: R-HSA-983168
Alternative Antibody Names Anti-Nedd8-Conjugating Enzyme Ube2f antibody
Anti-Nedd8 Carrier Protein Ube2f antibody
Anti-Nedd8 Protein Ligase Ube2f antibody
Anti-Nedd8-Conjugating Enzyme 2 antibody
Anti-Ring-Type E3 Nedd8 Transferase Ube2f antibody
Anti-Ubiquitin-Conjugating Enzyme E2 F antibody
Anti-UBE2F antibody
Anti-NCE2 antibody

Information sourced from Uniprot.org

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