Host: | Goat |
Applications: | Pep-ELISA |
Reactivity: | Human/Mouse/Dog |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Goat polyclonal antibody anti-NEBL/nebulette (Internal) is suitable for use in ELISA research applications. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: | 0.5 mg/mL |
Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 30kDa band in Human Brain (Frontal Cortex) lysates, but not in Human, Mouse and Rat Heart lysates after 1µg/ml antibody staining. This protein has |
Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: | NEBL |
Gene ID: | 10529 |
Uniprot ID: | NEBL_HUMAN |
Immunogen Region: | Internal |
Accession Number: | NP_998734.1; NP_001166955.1 |
Specificity: | This antibody is expected to recognize isoform 2 and isoform 3 (NP_998734.1; NP_001166955.1) only, but does not recognize isoform 1 (NP_006384.1). |
Immunogen Sequence: | ELQRLKRTQE |
Function | Binds to actin and plays an important role in the assembly of the Z-disk. May functionally link sarcomeric actin to the desmin intermediate filaments in the heart muscle sarcomeres. Isoform 2: May play a role in the assembly of focal adhesions. |
Protein Name | NebuletteActin-Binding Z-Disk Protein |
Cellular Localisation | Isoform 2: Cytoplasm |
Alternative Antibody Names | Anti-Nebulette antibodyAnti-Actin-Binding Z-Disk Protein antibodyAnti-NEBL antibodyAnti-C10orf113 antibodyAnti-LNEBL antibody |
Information sourced from Uniprot.org