• Immunofluorescence analysis of L929 cells using NDUFS8 Rabbit polyclonal antibody (STJ115001) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NDUFS8 Rabbit polyclonal antibody (STJ115001) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NDUFS8 antibody (35-210) (STJ115001)

SKU:
STJ115001

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFS8 (35-210) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFS8
Gene ID: 4728
Uniprot ID: NDUS8_HUMAN
Immunogen Region: 35-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 35-210 of human NDUFS8 (NP_002487.1).
Immunogen Sequence: TYKYVNMQDPEMDMKSVTDR AARTLLWTELFRGLGMTLSY LFREPATINYPFEKGPLSPR FRGEHALRRYPSGEERCIAC KLCEAICPAQAITIEAEPRA DGSRRTTRYDIDMTKCIYCG FCQEACPVDAIVEGPNFEFS TETHEELLYNKEKLLNNGDK WEAEIAANIQADYLYR
Tissue Specificity Expressed in all tissues with the highest level in heart and skeletal muscle and the lowest level in lung.
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.
Protein Name Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 8 - Mitochondrial
Complex I-23kd
Ci-23kd
Nadh-Ubiquinone Oxidoreductase 23 Kda Subunit
Tyky Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 8 - Mitochondrial antibody
Anti-Complex I-23kd antibody
Anti-Ci-23kd antibody
Anti-Nadh-Ubiquinone Oxidoreductase 23 Kda Subunit antibody
Anti-Tyky Subunit antibody
Anti-NDUFS8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance