• Immunofluorescence analysis of L929 cells using NDUFS5 Rabbit polyclonal antibody (STJ11104382) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts from wild type (WT) and NDUFS5 knockdown (KD) 293T cells, using NDUFS5 antibody (STJ11104382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates, using NDUFS5 Rabbit polyclonal antibody (STJ11104382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NDUFS5 antibody (1-106) (STJ11104382)

SKU:
STJ11104382

£129.50 - £639.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 5 (1-106) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFS5
Gene ID: 4725
Uniprot ID: NDUS5_HUMAN
Immunogen Region: 1-106
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-106 of human NDUFS5 (NP_004543.1).
Immunogen Sequence: MPFLDIQKRFGLNIDRWLTI QSGEQPYKMAGRCHAFEKEW IECAHGIGYTRAEKECKIEY DDFVECLLRQKTMRRAGTIR KQRDKLIKEGKYTPPPHHIG KGEPRP
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 5
Complex I-15 Kda
Ci-15 Kda
Nadh-Ubiquinone Oxidoreductase 15 Kda Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Mitochondrion Intermembrane Space
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 5 antibody
Anti-Complex I-15 Kda antibody
Anti-Ci-15 Kda antibody
Anti-Nadh-Ubiquinone Oxidoreductase 15 Kda Subunit antibody
Anti-NDUFS5 antibody

Information sourced from Uniprot.org

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