• Western blot analysis of extracts of various cell lines, using NDUFS4 antibody (STJ11104687) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using NDUFS4 antibody (STJ11104687) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NDUFS4 antibody (43-175) [S4687RM] (STJ11104687)

SKU:
STJ11104687

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 4-Mitochondrial (43-175) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4687RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFS4
Gene ID: 4724
Uniprot ID: NDUS4_HUMAN
Immunogen Region: 43-175
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 43-175 of human NDUFS4 (NP_002486.1).
Immunogen Sequence: AQDQTQDTQLITVDEKLDIT TLTGVPEEHIKTRKVRIFVP ARNNMQSGVNNTKKWKMEFD TRERWENPLMGWASTADPLS NMVLTFSTKEDAVSFAEKNG WSYDIEERKVPKPKSKSYGA NFSWNKRTRVSTK
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 4 - Mitochondrial
Complex I-18 Kda
Ci-18 Kda
Complex I-Aqdq
Ci-Aqdq
Nadh-Ubiquinone Oxidoreductase 18 Kda Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
The Interaction With Bcap31 Mediates Mitochondria Localization
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 4 - Mitochondrial antibody
Anti-Complex I-18 Kda antibody
Anti-Ci-18 Kda antibody
Anti-Complex I-Aqdq antibody
Anti-Ci-Aqdq antibody
Anti-Nadh-Ubiquinone Oxidoreductase 18 Kda Subunit antibody
Anti-NDUFS4 antibody

Information sourced from Uniprot.org

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