• Western blot analysis of extracts of various cell lines, using NDUFS3 antibody (STJ110319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunoprecipitation analysis of 600 Mu g extracts of Mouse kidney using 3 Mu g NDUFS3 antibody (STJ110319). Western blot was performed from the immunoprecipitate using NDUFS3 antibody (STJ110319) at a dilution of 1:1000.
  • Western blot analysis of extracts from normal (control) and NDUFS3 knockout (KO) 293T cells, using NDUFS3 antibody (STJ110319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using NDUFS3 Rabbit polyclonal antibody (STJ110319) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 90s.
  • Immunoprecipitation analysis of 600ug extracts of mouse kidney using 3ug NDUFS3 antibody (STJ110319). Western blot was performed from the immunoprecipitate using NDUFS3 antibody (STJ110319) at a dilition of 1:1000.

Anti-NDUFS3 antibody (37-264) (STJ110319)

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STJ110319

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFS3 (37-264) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFS3
Gene ID: 4722
Uniprot ID: NDUS3_HUMAN
Immunogen Region: 37-264
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 37-264 of human NDUFS3 (NP_004542.1).
Immunogen Sequence: ESAGADTRPTVRPRNDVAHK QLSAFGEYVAEILPKYVQQV QVSCFNELEVCIHPDGVIPV LTFLRDHTNAQFKSLVDLTA VDVPTRQNRFEIVYNLLSLR FNSRIRVKTYTDELTPIESA VSVFKAANWYEREIWDMFGV FFANHPDLRRILTDYGFEGH PFRKDFPLSGYVELRYDDEV KRVVAEPVELAQEFRKFDLN SPWEAFPVYRQPPESLKLEA GDKKPDAK
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.
Protein Name Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 3 - Mitochondrial
Complex I-30kd
Ci-30kd
Nadh-Ubiquinone Oxidoreductase 30 Kda Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Reactome: R-HSA-9013408
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 3 - Mitochondrial antibody
Anti-Complex I-30kd antibody
Anti-Ci-30kd antibody
Anti-Nadh-Ubiquinone Oxidoreductase 30 Kda Subunit antibody
Anti-NDUFS3 antibody

Information sourced from Uniprot.org

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