| Short Description : | Rabbit polyclonal anti-NADH dehydrogenase 1 subunit C2 (51-100) for use in IHC, IF and ELISA in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | IHC/IF/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range: | IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NDUFC2 |
| Gene ID: | 4718 |
| Uniprot ID: | NDUC2_HUMAN |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human NDUC2. AA range:51-100 |
| Immunogen Region: | 51-100 |
| Specificity: | NDUFC2 Polyclonal Antibody detects endogenous levels of NDUFC2 protein. |
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis but required for the complex assembly. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Protein Name | Nadh Dehydrogenase Ubiquinone 1 Subunit C2Complex I-B14.5bCi-B14.5bHuman Lung Cancer Oncogene 1 ProteinHlc-1Nadh-Ubiquinone Oxidoreductase Subunit B14.5b |
| Database Links | Reactome: R-HSA-611105Reactome: R-HSA-6798695Reactome: R-HSA-6799198 |
| Cellular Localisation | Mitochondrion Inner MembraneSingle-Pass Membrane ProteinMatrix Side |
| Alternative Antibody Names | Anti-Nadh Dehydrogenase Ubiquinone 1 Subunit C2 antibodyAnti-Complex I-B14.5b antibodyAnti-Ci-B14.5b antibodyAnti-Human Lung Cancer Oncogene 1 Protein antibodyAnti-Hlc-1 antibodyAnti-Nadh-Ubiquinone Oxidoreductase Subunit B14.5b antibodyAnti-NDUFC2 antibodyAnti-HLC1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and NDUFC2 knockout (KO) HeLa cells, using [KO Validated] NDUFC2 Rabbit polyclonal antibody (STJ117267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/96229/378614/STJ117267_1__93153.1713146239.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NDUFC2 knockout (KO) HeLa cells, using [KO Validated] NDUFC2 Rabbit polyclonal antibody (STJ117267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
