Western blot analysis of various lysates, using NDUFC2 Rabbit monoclonal antibody (STJ11104184) at1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-NDUFC2 antibody (1-119) [S4184RM] (STJ11104184)

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STJ11104184
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S4184RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFC2
Gene ID: 4718
Uniprot ID: NDUC2_HUMAN
Immunogen Region: 1-119
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-119 of human NDUFC2 (NP_004540.1).
Immunogen Sequence: MIARRNPEPLRFLPDEARSL PPPKLTDPRLLYIGFLGYCS GLIDNLIRRRPIATAGLHRQ LLYITAFFFAGYYLVKREDY LYAVRDREMFGYMKLHPEDF PEEDKKTYGEIFEKFHPIR
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis but required for the complex assembly. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Subunit C2
Complex I-B14.5b
Ci-B14.5b
Human Lung Cancer Oncogene 1 Protein
Hlc-1
Nadh-Ubiquinone Oxidoreductase Subunit B14.5b
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6798695
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Subunit C2 antibody
Anti-Complex I-B14.5b antibody
Anti-Ci-B14.5b antibody
Anti-Human Lung Cancer Oncogene 1 Protein antibody
Anti-Hlc-1 antibody
Anti-Nadh-Ubiquinone Oxidoreductase Subunit B14.5b antibody
Anti-NDUFC2 antibody
Anti-HLC1 antibody

Information sourced from Uniprot.org

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