• Western blot analysis of extracts of various cell lines, using NDUFB8 rabbit monoclonal antibody (STJ11103099) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using NDUFB8 rabbit monoclonal antibody (STJ11103099) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using NDUFB8 rabbit monoclonal antibody (STJ11103099) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using NDUFB8 rabbit monoclonal antibody (STJ11103099) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using NDUFB8 rabbit monoclonal antibody (STJ11103099) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using NDUFB8 rabbit monoclonal antibody (STJ11103099) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using NDUFB8 rabbit monoclonal antibody (STJ11103099) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using NDUFB8 rabbit monoclonal antibody (STJ11103099) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U-2 OS cells using NDUFB8 rabbit monoclonal antibody (STJ11103099) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Anti-NDUFB8 antibody (1-150) [S9MR] (STJ11103099)

Anti-NDUFB8 antibody (1-150) [S9MR] (STJ11103099)

SKU:
STJ11103099

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-NDUFB8 (1-150) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:3000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFB8
Gene ID: 4714
Uniprot ID: NDUB8_HUMAN
Immunogen Region: 1-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human NDUFB8 (O95169).
Immunogen Sequence: MAVARAGVLGVQWLQRASRN VMPLGARTASHMTKDMFPGP YPRTPEERAAAAKKYNMRVE DYEPYPDDGMGYGDYPKLPD RSQHERDPWYSWDQPGLRLN WGEPMHWHLDMYNRNRVDTS PTPVSWHVMCMQLFGFLAFM IFMCWVGDVY
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 8 - Mitochondrial
Complex I-Ashi
Ci-Ashi
Nadh-Ubiquinone Oxidoreductase Ashi Subunit
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 8 - Mitochondrial antibody
Anti-Complex I-Ashi antibody
Anti-Ci-Ashi antibody
Anti-Nadh-Ubiquinone Oxidoreductase Ashi Subunit antibody
Anti-NDUFB8 antibody

Information sourced from Uniprot.org

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