| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-NADH dehydrogenase 1 beta subcomplex subunit 10 (63-112) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NDUFB10 |
| Gene ID: | 4716 |
| Uniprot ID: | NDUBA_HUMAN |
| Immunogen Region: | 63-112 |
| Specificity: | NDUFB10 Polyclonal Antibody detects endogenous levels of NDUFB10 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human NDUFB10. AA range:63-112 |
| Post Translational Modifications | The formation of intramolecular disulfide bonds is assisted by CHCHD4 and ensures folding, import into the mitochondrion and is required for the function in mitochondrial respiratory chain complex I assembly. |
| Function | Accessory subunit that is involved in the functional assembly of the mitochondrial respiratory chain complex I. Complex I has an NADH dehydrogenase activity with ubiquinone as an immediate electron acceptor and mediates the transfer of electrons from NADH to the respiratory chain. |
| Protein Name | Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 10Complex I-PdswCi-PdswNadh-Ubiquinone Oxidoreductase Pdsw Subunit |
| Database Links | Reactome: R-HSA-611105Reactome: R-HSA-6799198 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinMatrix Side |
| Alternative Antibody Names | Anti-Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 10 antibodyAnti-Complex I-Pdsw antibodyAnti-Ci-Pdsw antibodyAnti-Nadh-Ubiquinone Oxidoreductase Pdsw Subunit antibodyAnti-NDUFB10 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and MRPL53 knockdown (KD) HeLa cells using [KD Validated] MRPL53 Rabbit polyclonal antibody (STJ11105622) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/178266/370594/STJ11105622_1__58411.1713136541.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MRPL53 knockdown (KD) HeLa cells using [KD Validated] MRPL53 Rabbit polyclonal antibody (STJ11105622) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
