| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-NDUFA7 (27-38) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 14kDa band observed in Human Heart lysates (calculated MW of 12.6kDa according to NP_004992.2). Recommended concentration: 0.1-0.3µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | NDUFA7 |
| Gene ID: | 4701 |
| Uniprot ID: | NDUA7_HUMAN |
| Immunogen Region: | 27-38 |
| Accession Number: | NP_004992.2 |
| Immunogen Sequence: | RYQEISKRTQPP |
| Protein Name | Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 7Complex I-B14.5aCi-B14.5aNadh-Ubiquinone Oxidoreductase Subunit B14.5a |
| Database Links | Reactome: R-HSA-611105Reactome: R-HSA-6799198 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinMatrix Side |
| Alternative Antibody Names | Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 7 antibodyAnti-Complex I-B14.5a antibodyAnti-Ci-B14.5a antibodyAnti-Nadh-Ubiquinone Oxidoreductase Subunit B14.5a antibodyAnti-NDUFA7 antibody |
Information sourced from Uniprot.org
![Western blot analysis of various lysates using [KO Validated] NDUFA7 Rabbit pAb (STJ110739) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/88942/405386/STJ110739_1__25973.1744891138.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] NDUFA7 Rabbit pAb (STJ110739) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.")
