• Western blot analysis of extracts of various cell lines, using NDUFA7 antibody (STJ110739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts from normal (control) and NDUFA7 knockout (KO) 293T cells, using NDUFA7 antibody (STJ110739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts from normal (control) and NDUFA7 knockout (KO) 293T cells, using NDUFA7 antibody (STJ110739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of various cell lines, using NDUFA7 antibody (STJ110739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NDUFA7 antibody (1-113) (STJ110739)

SKU:
STJ110739

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFA7 (1-113) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFA7
Gene ID: 4701
Uniprot ID: NDUA7_HUMAN
Immunogen Region: 1-113
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human NDUFA7 (NP_004992.2).
Immunogen Sequence: MASATRLIQRLRNWASGHDL QGKLQLRYQEISKRTQPPPK LPVGPSHKLSNNYYCTRDGR RESVPPSIIMSSQKALVSGK PAESSAVAATEKKAVTPAPP IKRWELSSDQPYL
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 7
Complex I-B14.5a
Ci-B14.5a
Nadh-Ubiquinone Oxidoreductase Subunit B14.5a
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 7 antibody
Anti-Complex I-B14.5a antibody
Anti-Ci-B14.5a antibody
Anti-Nadh-Ubiquinone Oxidoreductase Subunit B14.5a antibody
Anti-NDUFA7 antibody

Information sourced from Uniprot.org

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