| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NDC80 |
| Gene ID: |
10403 |
| Uniprot ID: |
NDC80_HUMAN |
| Immunogen Region: |
343-642 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 343-642 of human HEC1/HEC1/NDC80 (NP_006092.1). |
| Immunogen Sequence: |
ETIKQENTRLQNIIDNQKYS VADIERINHERNELQQTINK LTKDLEAEQQKLWNEELKYA RGKEAIETQLAEYHKLARKL KLIPKGAENSKGYDFEIKFN PEAGANCLVKYRAQVYVPLK ELLNETEEEINKALNKKMGL EDTLEQLNAMITESKRSVRT LKEEVQKLDDLYQQKIKEAE EEDEKCASELESLEKHKHLL ESTVNQGLSEAMNELDAVQR EYQLVVQTTTEERRKVGNN |
| Post Translational Modifications | Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. Also phosphorylated by AURKA and AURKB. Acetylated at Lys-53 and Lys-59 by KAT5 during mitosis, promoting robust kinetochore-microtubule attachment. Deacetylated by SIRT1. |
| Function | Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules. |
| Protein Name | Kinetochore Protein Ndc80 HomologHighly Expressed In Cancer ProteinKinetochore Protein Hec1Hshec1Kinetochore-Associated Protein 2Retinoblastoma-Associated Protein Hec |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreLocalizes To Kinetochores From Late Prophase To AnaphaseLocalizes Specifically To The Outer Plate Of The Kinetochore |
| Alternative Antibody Names | Anti-Kinetochore Protein Ndc80 Homolog antibodyAnti-Highly Expressed In Cancer Protein antibodyAnti-Kinetochore Protein Hec1 antibodyAnti-Hshec1 antibodyAnti-Kinetochore-Associated Protein 2 antibodyAnti-Retinoblastoma-Associated Protein Hec antibodyAnti-NDC80 antibodyAnti-HEC antibodyAnti-HEC1 antibodyAnti-KNTC2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
