Anti-NCK2 antibody (1-380) (STJ110061)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NCK2 (1-380) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NCK2 |
| Gene ID | 8440 |
| Uniprot ID | NCK2_HUMAN |
| Immunogen Region | 1-380 |
| Immunogen Sequence | MTEEVIVIAKWDYTAQQDQE LDIKKNERLWLLDDSKTWWR VRNAANRTGYVPSNYVERKN SLKKGSLVKNLKDTLGLGKT RRKTSARDASPTPSTDAEYP ANGSGADRIYDLNIPAFVKF AYVAEREDELSLVKGSRVTV MEKCSDGWWRGSYNGQIGWF PSNYVLEEVDEAAAESPSFL SLRKGASLSNGQGSRVLHVV QTLYPFSSVTEEELNFEKGE TMEVIEKPENDPEWWKCKN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-380 of human NCK2 (NP_003572.2). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylated. |
| Function | Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. |
| Protein Name | Cytoplasmic Protein Nck2Growth Factor Receptor-Bound Protein 4Nck Adaptor Protein 2Nck-2Sh2/Sh3 Adaptor Protein Nck-Beta |
| Database Links | Reactome: R-HSA-186763Reactome: R-HSA-373753Reactome: R-HSA-3928664Reactome: R-HSA-428540Reactome: R-HSA-4420097Reactome: R-HSA-8985801Reactome: R-HSA-9013420Reactome: R-HSA-9013424 |
| Cellular Localisation | CytoplasmEndoplasmic Reticulum |
| Alternative Antibody Names | Anti-Cytoplasmic Protein Nck2 antibodyAnti-Growth Factor Receptor-Bound Protein 4 antibodyAnti-Nck Adaptor Protein 2 antibodyAnti-Nck-2 antibodyAnti-Sh2/Sh3 Adaptor Protein Nck-Beta antibodyAnti-NCK2 antibodyAnti-GRB4 antibody |
Information sourced from Uniprot.org