| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NCK2 |
| Gene ID: | 8440 |
| Uniprot ID: | NCK2_HUMAN |
| Immunogen Region: | 1-380 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-380 of human NCK2 (NP_003572.2). |
| Immunogen Sequence: | MTEEVIVIAKWDYTAQQDQE LDIKKNERLWLLDDSKTWWR VRNAANRTGYVPSNYVERKN SLKKGSLVKNLKDTLGLGKT RRKTSARDASPTPSTDAEYP ANGSGADRIYDLNIPAFVKF AYVAEREDELSLVKGSRVTV MEKCSDGWWRGSYNGQIGWF PSNYVLEEVDEAAAESPSFL SLRKGASLSNGQGSRVLHVV QTLYPFSSVTEEELNFEKGE TMEVIEKPENDPEWWKCKN |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylated. |
| Function | Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. |
| Protein Name | Cytoplasmic Protein Nck2Growth Factor Receptor-Bound Protein 4Nck Adaptor Protein 2Nck-2Sh2/Sh3 Adaptor Protein Nck-Beta |
| Database Links | Reactome: R-HSA-186763Reactome: R-HSA-373753Reactome: R-HSA-3928664Reactome: R-HSA-428540Reactome: R-HSA-4420097Reactome: R-HSA-8985801Reactome: R-HSA-9013420Reactome: R-HSA-9013424 |
| Cellular Localisation | CytoplasmEndoplasmic Reticulum |
| Alternative Antibody Names | Anti-Cytoplasmic Protein Nck2 antibodyAnti-Growth Factor Receptor-Bound Protein 4 antibodyAnti-Nck Adaptor Protein 2 antibodyAnti-Nck-2 antibodyAnti-Sh2/Sh3 Adaptor Protein Nck-Beta antibodyAnti-NCK2 antibodyAnti-GRB4 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and PAK2 knockout (KO) HeLa cells, using [KO Validated] PAK2 Rabbit polyclonal antibody (STJ11100066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89136/357585/STJ11100066_1__93634.1713121719.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PAK2 knockout (KO) HeLa cells, using [KO Validated] PAK2 Rabbit polyclonal antibody (STJ11100066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
