• STJ71228 (0. 5µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • HEK293 overexpressing P40PHOX (RC201191) and probed with STJ71228 (mock transfection in first lane) , tested by Origene.
  • STJ71228 (2. 5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-NCF4/P40PHOX antibody (Internal) (STJ71228)

SKU:
STJ71228-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-NCF4/P40PHOX (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-2.5µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NCF4
Gene ID: 4689
Uniprot ID: NCF4_HUMAN
Immunogen Region: Internal
Accession Number: NP_000622.2; NP_038202.1
Specificity: This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).
Immunogen Sequence: KDFPEEDDPTN
Function Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
Protein Name Neutrophil Cytosol Factor 4
Ncf-4
Neutrophil Nadph Oxidase Factor 4
Sh3 And Px Domain-Containing Protein 4
P40-Phox
P40phox
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-1236973
Reactome: R-HSA-3299685
Reactome: R-HSA-4420097
Reactome: R-HSA-5668599
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013423
Cellular Localisation Cytoplasm
Cytosol
Endosome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Membrane
Alternative Antibody Names Anti-Neutrophil Cytosol Factor 4 antibody
Anti-Ncf-4 antibody
Anti-Neutrophil Nadph Oxidase Factor 4 antibody
Anti-Sh3 And Px Domain-Containing Protein 4 antibody
Anti-P40-Phox antibody
Anti-P40phox antibody
Anti-NCF4 antibody
Anti-SH3PXD4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance