• Immunoprecipitation analysis of 300 Mu g extracts of THP-1 cells using 3 Mu g NCF4/p40-phox antibody (STJ11102723). Western blot was performed from the immunoprecipitate using NCF4/p40-phox antibody (STJ11102723) at a dilution of 1:1000.
  • Immunohistochemistry analysis of NCF4/p40-phox in paraffin-embedded human colon carcinoma using NCF4/p40-phox Rabbit monoclonal antibody (STJ11102723) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse thymus, using NCF4/p40-phox Rabbit monoclonal antibody (STJ11102723) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of lysates from THP-1 cells, using NCF4/p40-phox Rabbit monoclonal antibody (STJ11102723) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NCF4/p40-phox antibody (240-339) [S3MR] (STJ11102723)

SKU:
STJ11102723

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-NCF4/p40-phox (240-339) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCF4
Gene ID: 4689
Uniprot ID: NCF4_HUMAN
Immunogen Region: 240-339
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 240-339 of human NCF4/p40-phox (Q15080).
Immunogen Sequence: LRCYYYEDTISTIKDIAVEE DLSSTPLLKDLLELTRREFQ REDIALNYRDAEGDLVRLLS DEDVALMVRQARGLPSQKRL FPWKLHITQKDNYRVYNTMP
Tissue Specificity Expression is restricted to hematopoietic cells.
Function Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
Protein Name Neutrophil Cytosol Factor 4
Ncf-4
Neutrophil Nadph Oxidase Factor 4
Sh3 And Px Domain-Containing Protein 4
P40-Phox
P40phox
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-1236973
Reactome: R-HSA-3299685
Reactome: R-HSA-4420097
Reactome: R-HSA-5668599
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013423
Cellular Localisation Cytoplasm
Cytosol
Endosome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Membrane
Alternative Antibody Names Anti-Neutrophil Cytosol Factor 4 antibody
Anti-Ncf-4 antibody
Anti-Neutrophil Nadph Oxidase Factor 4 antibody
Anti-Sh3 And Px Domain-Containing Protein 4 antibody
Anti-P40-Phox antibody
Anti-P40phox antibody
Anti-NCF4 antibody
Anti-SH3PXD4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance