• Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using NOXA2/p67phox Rabbit polyclonal antibody (STJ24699) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NOXA2/p67phox antibody (STJ24699) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-NCF2 antibody (227-526) (STJ24699)

SKU:
STJ24699

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NOXA2/P67phox (227-526) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCF2
Gene ID: 4688
Uniprot ID: NCF2_HUMAN
Immunogen Region: 227-526
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 227-526 of human NOXA2/p67phox (NP_000424.2).
Immunogen Sequence: PPPRPKTPEIFRALEGEAHR VLFGFVPETKEELQVMPGNI VFVLKKGNDNWATVMFNGQK GLVPCNYLEPVELRIHPQQQ PQEESSPQSDIPAPPSSKAP GRPQLSPGQKQKEEPKEVKL SVPMPYTLKVHYKYTVVMKT QPGLPYSQVRDMVSKKLELR LEHTKLSYRPRDSNELVPLS EDSMKDAWGQVKNYCLTLWC ENTVGDQGFPDEPKESEKAD ANNQTTEPQLKKGSQVEAL
Function NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Protein Name Neutrophil Cytosol Factor 2
Ncf-2
67 Kda Neutrophil Oxidase Factor
Nadph Oxidase Activator 2
Neutrophil Nadph Oxidase Factor 2
P67-Phox
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-1236973
Reactome: R-HSA-3299685
Reactome: R-HSA-4420097
Reactome: R-HSA-5668599
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013423
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Neutrophil Cytosol Factor 2 antibody
Anti-Ncf-2 antibody
Anti-67 Kda Neutrophil Oxidase Factor antibody
Anti-Nadph Oxidase Activator 2 antibody
Anti-Neutrophil Nadph Oxidase Factor 2 antibody
Anti-P67-Phox antibody
Anti-NCF2 antibody
Anti-NOXA2 antibody
Anti-P67PHOX antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance