| Tissue Specificity | Ubiquitous. |
| Function | Catalyzes the condensation of phosphoenolpyruvate (PEP) and N-acetylmannosamine 6-phosphate (ManNAc-6-P) to synthesize N-acetylneuraminate-9-phosphate (Neu5Ac-9-P). Also catalyzes the condensation of PEP and D-mannose 6-phosphate (Man-6-P) to produce 3-deoxy-D-glycero-beta-D-galacto-non-2-ulopyranosonate 9-phosphate (KDN-9-P). Neu5Ac-9-P and KDN-9-P are the phosphorylated forms of sialic acids N-acetylneuraminic acid (Neu5Ac) and deaminoneuraminic acid (KDN), respectively. Required for brain and skeletal development. |
| Protein Name | N-Acetylneuraminate-9-Phosphate Synthase3-Deoxy-D-Glycero-D-Galacto-Nononate 9-Phosphate SynthaseN-Acetylneuraminic Acid Phosphate SynthaseNansSialic Acid Phosphate SynthaseSialic Acid Synthase |
| Database Links | Reactome: R-HSA-4085001 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-N-Acetylneuraminate-9-Phosphate Synthase antibodyAnti-3-Deoxy-D-Glycero-D-Galacto-Nononate 9-Phosphate Synthase antibodyAnti-N-Acetylneuraminic Acid Phosphate Synthase antibodyAnti-Nans antibodyAnti-Sialic Acid Phosphate Synthase antibodyAnti-Sialic Acid Synthase antibodyAnti-NANS antibodyAnti-SAS antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and NANS knockout (KO) 293T cells, using [KO Validated] NANS Rabbit polyclonal antibody (STJ11100751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89689/358177/STJ11100751_1__56006.1713122486.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NANS knockout (KO) 293T cells, using [KO Validated] NANS Rabbit polyclonal antibody (STJ11100751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and NANS knockout (KO) 293T cells, using [KO Validated] NANS Rabbit polyclonal antibody (STJ11100751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89689/358177/STJ11100751_1__56006.1713122486.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NANS knockout (KO) 293T cells, using [KO Validated] NANS Rabbit polyclonal antibody (STJ11100751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunofluorescence analysis of MCF7 cells using [KD Validated] SCD Rabbit polyclonal antibody (STJ11105577) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/178221/370505/STJ11105577_1__69759.1713136460.jpg?c=1 "Immunofluorescence analysis of MCF7 cells using [KD Validated] SCD Rabbit polyclonal antibody (STJ11105577) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
