![Western blot analysis of lysates from wild type (WT) and NANS knockout (KO) 293T cells, using [KO Validated] NANS Rabbit polyclonal antibody (STJ11100751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89689/358177/STJ11100751_1__56006.1713122486.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NANS knockout (KO) 293T cells, using [KO Validated] NANS Rabbit polyclonal antibody (STJ11100751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and NANS knockout (KO) 293T cells, using [KO Validated] NANS Rabbit polyclonal antibody (STJ11100751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89689/358177/STJ11100751_1__56006.1713122486.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NANS knockout (KO) 293T cells, using [KO Validated] NANS Rabbit polyclonal antibody (STJ11100751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NANS |
| Gene ID: | 54187 |
| Uniprot ID: | SIAS_HUMAN |
| Immunogen Region: | 260-359 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 260-359 of human NANS (NP_061819.2). |
| Immunogen Sequence: | AELVRSVRLVERALGSPTKQ LLPCEMACNEKLGKSVVAKV KIPEGTILTMDMLTVKVGEP KGYPPEDIFNLVGKKVLVTV EEDDTIMEELVDNHGKKIKS |
| Tissue Specificity | Ubiquitous. |
| Function | Produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN). Can also use N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively. |
| Protein Name | Sialic Acid SynthaseN-Acetylneuraminate SynthaseN-Acetylneuraminate-9-Phosphate SynthaseN-Acetylneuraminic Acid Phosphate SynthaseN-Acetylneuraminic Acid Synthase |
| Database Links | Reactome: R-HSA-4085001 |
| Alternative Antibody Names | Anti-Sialic Acid Synthase antibodyAnti-N-Acetylneuraminate Synthase antibodyAnti-N-Acetylneuraminate-9-Phosphate Synthase antibodyAnti-N-Acetylneuraminic Acid Phosphate Synthase antibodyAnti-N-Acetylneuraminic Acid Synthase antibodyAnti-NANS antibodyAnti-SAS antibody |
Information sourced from Uniprot.org