Anti-Nampt antibody [R05-4C4] (STJA0035403)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human Visfatin
General Information
| Short Description | Rabbit monoclonal anti-Nampt for use in WB, IHC-F, IHC-P, ICC, IF and IP in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF/IP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R05-4C4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | NAMPT |
| Gene ID | 10135 |
| Uniprot ID | NAMPT_HUMAN |
| Immunogen | A synthetic peptide of human Visfatin |
Additional Info
| Tissue Specificity | Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues. |
| Function | Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression. |
| Protein Name | Nicotinamide PhosphoribosyltransferaseNamprtaseNamptPre-B-Cell Colony-Enhancing Factor 1Pre-B Cell-Enhancing FactorVisfatin |
| Database Links | Reactome: R-HSA-1368108Reactome: R-HSA-197264Reactome: R-HSA-9768919 |
| Cellular Localisation | NucleusCytoplasmSecretedUnder Non-Inflammatory ConditionsVisfatin Predominantly Exhibits A Granular Pattern Within The NucleusSecreted By Endothelial Cells Upon Il-1beta StimulationAbundantly Secreted In MilkReaching 100-Fold Higher Concentrations Compared To Maternal Serum |
| Alternative Antibody Names | Anti-Nicotinamide Phosphoribosyltransferase antibodyAnti-Namprtase antibodyAnti-Nampt antibodyAnti-Pre-B-Cell Colony-Enhancing Factor 1 antibodyAnti-Pre-B Cell-Enhancing Factor antibodyAnti-Visfatin antibodyAnti-NAMPT antibodyAnti-PBEF antibodyAnti-PBEF1 antibody |
Information sourced from Uniprot.org