• Western blot analysis of extracts of various cell lines, using NAGK antibody (STJ111552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of various cell lines, using NAGK antibody (STJ111552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NAGK antibody (50-210) (STJ111552)

SKU:
STJ111552

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NAGK (50-210) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NAGK
Gene ID: 55577
Uniprot ID: NAGK_HUMAN
Immunogen Region: 50-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-210 of human NAGK (NP_060037.3).
Immunogen Sequence: IYGGVEGGGTRSEVLLVSED GKILAEADGLSTNHWLIGTD KCVERINEMVNRAKRKAGVD PLVPLRSLGLSLSGGDQEDA GRILIEELRDRFPYLSESYL ITTDAAGSIATATPDGGVVL ISGTGSNCRLINPDGSESGC GGWGHMMGDEGSAYWIAHQA V
Tissue Specificity Ubiquitous.
Function Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway: although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded. Also has N-acetylmannosamine (ManNAc) kinase activity. Also involved in innate immunity by promoting detection of bacterial peptidoglycan by NOD2: acts by catalyzing phosphorylation of muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, to generate 6-O-phospho-muramyl dipeptide, which acts as a direct ligand for NOD2.
Protein Name N-Acetyl-D-Glucosamine Kinase
N-Acetylglucosamine Kinase
Glcnac Kinase
Muramyl Dipeptide Kinase
N-Acetyl-D-Mannosamine Kinase
Database Links Reactome: R-HSA-446210
Alternative Antibody Names Anti-N-Acetyl-D-Glucosamine Kinase antibody
Anti-N-Acetylglucosamine Kinase antibody
Anti-Glcnac Kinase antibody
Anti-Muramyl Dipeptide Kinase antibody
Anti-N-Acetyl-D-Mannosamine Kinase antibody
Anti-NAGK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance