• Immunohistochemistry analysis of N6AMT1/HEMK2 in paraffin-embedded human thyroid cancer tissue using N6AMT1/HEMK2 Rabbit polyclonal antibody (STJ11104540) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates, using N6AMT1 Rabbit polyclonal antibody (STJ11104540) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer:3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: Q120s.

Anti-N6AMT1 antibody (1-214) (STJ11104540)

SKU:
STJ11104540

£112.50 - £522.50
Current Stock:
Host: rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Methyltransferase N6amt1 (1-214) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: N6AMT1
Gene ID: 29104
Uniprot ID: N6MT1_HUMAN
Immunogen Region: 1-214
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-214 of human N6AMT1/HEMK2 (NP_037372.4).
Immunogen Sequence: MAGENFATPFHGHVGRGAFS DVYEPAEDTFLLLDALEAAA AELAGVEICLEVGSGSGVVS AFLASMIGPQALYMCTDINP EAAACTLETARCNKVHIQPV ITDLVKGLLPRLTEKVDLLV FNPPYVVTPPQEVGSHGIEA AWAGGRNGREVMDRFFPLVP DLLSPRGLFYLVTIKENNPE EILKIMKTKGLQGTTALSRQ AGQETLSVLKFTKS
Tissue Specificity Widely expressed, with highest expression in parathyroid and pituitary glands, followed by adrenal gland and kidney, and lowest expression in leukocytes and mammary gland.
Post Translational Modifications Isoform 1: Ubiquitinated, leading to its degradation by the proteasome. Isoform 2: Ubiquitinated, leading to its degradation by the proteasome.
Function Methyltransferase that can methylate proteins and, to a lower extent, arsenic. Catalytic subunit of a heterodimer with TRMT112, which monomethylates 'Lys-12' of histone H4 (H4K12me1), a modification present at the promoters of numerous genes encoding cell cycle regulators. Catalytic subunit of a heterodimer with TRMT112, which catalyzes N5-methylation of Glu residue of proteins with a Gly-Gln-Xaa-Xaa-Xaa-Arg motif. Methylates ETF1 on 'Gln-185'.ETF1 needs to be complexed to ERF3 in its GTP-bound form to be efficiently methylated. May also play a role in the modulation of arsenic-induced toxicity by mediating the conversion of monomethylarsonous acid (3+) into the less toxic dimethylarsonic acid. It however only plays a limited role in arsenic metabolism compared with AS3MT.
Protein Name Methyltransferase N6amt1
Hemk Methyltransferase Family Member 2
M.hsahemk2p
Lysine N-Methyltransferase 9
Methylarsonite Methyltransferase N6amt1
Protein N(5-Glutamine Methyltransferase
Database Links Reactome: R-HSA-156581
Reactome: R-HSA-72764
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Methyltransferase N6amt1 antibody
Anti-Hemk Methyltransferase Family Member 2 antibody
Anti-M.hsahemk2p antibody
Anti-Lysine N-Methyltransferase 9 antibody
Anti-Methylarsonite Methyltransferase N6amt1 antibody
Anti-Protein N(5-Glutamine Methyltransferase antibody
Anti-N6AMT1 antibody
Anti-C21orf127 antibody
Anti-HEMK2 antibody
Anti-KMT9 antibody
Anti-PRED28 antibody

Information sourced from Uniprot.org

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