• Human rhabdomyosarcoma stained with anti-Myogenin antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells.

Anti-Myogenin antibody [ZM149] (STJ180362)

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STJ180362

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Host: Mouse
Applications: IHC-P
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Myogenin is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: ZM149
Conjugation: Unconjugated
Isotype: IgG1/Kappa
Formulation: Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin
Purification: Affinity purified
Dilution Range: 1:100‐200
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Gene Symbol: MYOG
Gene ID: 4656
Uniprot ID: MYOG_HUMAN
Specificity: Positive control: Rhabdomyosarcoma or Wilm's tumor
Immunogen: Human myogenin recombinant protein
Post Translational Modifications Phosphorylated by CAMK2G on threonine and serine amino acids in a muscle activity-dependent manner. Phosphorylation of Thr-87 impairs both DNA-binding and trans-activation functions in contracting muscles.
Function Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth.phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts.
Protein Name Myogenin
Class C Basic Helix-Loop-Helix Protein 3
Bhlhc3
Myogenic Factor 4
Myf-4
Database Links Reactome: R-HSA-525793
Cellular Localisation Nucleus
Recruited To Late Myogenic Gene Promoter Regulatory Sequences With Smarca4/Brg1/Baf190a And Swi/Snf Chromatin-Remodeling Enzymes To Promote Chromatin-Remodeling And Transcription Initiation In Developing Embryos
Alternative Antibody Names Anti-Myogenin antibody
Anti-Class C Basic Helix-Loop-Helix Protein 3 antibody
Anti-Bhlhc3 antibody
Anti-Myogenic Factor 4 antibody
Anti-Myf-4 antibody
Anti-MYOG antibody
Anti-BHLHC3 antibody
Anti-MYF4 antibody

Information sourced from Uniprot.org

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