| Host: |
Mouse |
| Applications: |
IHC-P |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Myogenin is suitable for use in Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
F5D |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1k |
| Formulation: |
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide |
| Dilution Range: |
IHC 1:25 – 1:100 |
| Storage Instruction: |
Store at 2-8°C upon receipt. |
| Post Translational Modifications | Phosphorylated by CAMK2G on threonine and serine amino acids in a muscle activity-dependent manner. Phosphorylation of Thr-87 impairs both DNA-binding and trans-activation functions in contracting muscles. |
| Function | Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth.phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts. |
| Protein Name | MyogeninClass C Basic Helix-Loop-Helix Protein 3Bhlhc3Myogenic Factor 4Myf-4 |
| Database Links | Reactome: R-HSA-525793 |
| Cellular Localisation | NucleusRecruited To Late Myogenic Gene Promoter Regulatory Sequences With Smarca4/Brg1/Baf190a And Swi/Snf Chromatin-Remodeling Enzymes To Promote Chromatin-Remodeling And Transcription Initiation In Developing Embryos |
| Alternative Antibody Names | Anti-Myogenin antibodyAnti-Class C Basic Helix-Loop-Helix Protein 3 antibodyAnti-Bhlhc3 antibodyAnti-Myogenic Factor 4 antibodyAnti-Myf-4 antibodyAnti-MYOG antibodyAnti-BHLHC3 antibodyAnti-MYF4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
