| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MYOD1 |
| Gene ID: | 4654 |
| Uniprot ID: | MYOD1_HUMAN |
| Immunogen Region: | 226-300 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 226-300 of human MyoD1 (NP_002469.2). |
| Immunogen Sequence: | YEGAYYNEAPSEPRPGKSAA VSSLDCLSSIVERISTESPA APALLLADVPSESPPRRQEA AAPSEGESSGDPTQS |
| Post Translational Modifications | Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation. Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function. Ubiquitinated on the N-terminus.which is required for proteasomal degradation. Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity. |
| Function | Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. |
| Protein Name | Myoblast Determination Protein 1 Class C Basic Helix-Loop-Helix Protein 1 Bhlhc1 Myogenic Factor 3 Myf-3 |
| Database Links | Reactome: R-HSA-525793 Reactome: R-HSA-9839394 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Myoblast Determination Protein 1 antibody Anti-Class C Basic Helix-Loop-Helix Protein 1 antibody Anti-Bhlhc1 antibody Anti-Myogenic Factor 3 antibody Anti-Myf-3 antibody Anti-MYOD1 antibody Anti-BHLHC1 antibody Anti-MYF3 antibody Anti-MYOD antibody |
Information sourced from Uniprot.org