| Host: |
Rabbit |
| Applications: |
WB/IHC-P/ELISA |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MYOCD |
| Gene ID: |
93649 |
| Uniprot ID: |
MYCD_HUMAN |
| Immunogen Region: |
680-780 |
| Specificity: |
A synthetic peptide corresponding to a sequence within amino acids 680-780 of human MYOCD (NP_705832.1). |
| Immunogen Sequence: |
SQVCTAQMAGLHSSDKVGPK FSIPSPTFSKSSSAISEVTQ PPSYEDAVKQQMTRSQQMDE LLDVLIESGEMPADAREDHS CLQKVPKIPRSSRSPTAVLT K |
| Tissue Specificity | Expressed in the heart, aorta and bladder. Expressed in smooth muscle cell-containing tissues: stomach, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle. |
| Post Translational Modifications | Ubiquitinated.by STUB1/CHIP at the C-terminus, leading to its degradation by the proteasome. Phosphorylation by GSK3B is required for STUB1/CHIP-mediated ubiquitination. Phosphorylation negatively regulates the intrinsic myocardin transcriptional activity. Phosphorylated.by GSK3B. |
| Function | Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis, urinary bladder development, and differentiation of the smooth muscle cell lineage (myogenesis). Positively regulates the transcription of genes involved in vascular smooth muscle contraction. |
| Protein Name | Myocardin |
| Database Links | Reactome: R-HSA-9733709 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Myocardin antibodyAnti-MYOCD antibodyAnti-MYCD antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
