• Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-MYO9B antibody (304-353) (STJ94314)

SKU:
STJ94314

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Unconventional myosin-IXb (304-353) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-p 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYO9B
Gene ID: 4650
Uniprot ID: MYO9B_HUMAN
Immunogen Region: 304-353
Specificity: Myosin IXb Polyclonal Antibody detects endogenous levels of Myosin IXb protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human MYO9B. AA range:304-353
Tissue Specificity Detected in peripheral blood leukocytes (at protein level). Expressed predominantly in peripheral blood leukocytes and at lower levels, in thymus, spleen, testis, prostate, ovary, brain, small intestine and lung.
Function Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions. Also acts as a GTPase activator for RHOA. Plays a role in the regulation of cell migration via its role as RHOA GTPase activator. This is regulated by its interaction with the SLIT2 receptor ROBO1.interaction with ROBO1 impairs interaction with RHOA and subsequent activation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA.
Protein Name Unconventional Myosin-Ixb
Unconventional Myosin-9b
Database Links Reactome: R-HSA-2029482
Reactome: R-HSA-8980692
Reactome: R-HSA-8985586
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9035034
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cell Cortex
Perinuclear Region
Cytoskeleton
In Undifferentiated Cells Colocalizes With F-Actin In The Cell Periphery While In Differentiated Cells Its Localization Is Cytoplasmic With The Highest Levels In The Perinuclear Region
Alternative Antibody Names Anti-Unconventional Myosin-Ixb antibody
Anti-Unconventional Myosin-9b antibody
Anti-MYO9B antibody
Anti-MYR5 antibody

Information sourced from Uniprot.org

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