Anti-MYLIP antibody (266-445) (STJ29939)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MYLIP (266-445) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MYLIP |
| Gene ID | 29116 |
| Uniprot ID | MYLIP_HUMAN |
| Immunogen Region | 266-445 |
| Immunogen Sequence | RAITETHAFYRCDTVTSAVM MQYSRDLKGHLASLFLNENI NLGKKYVFDIKRTSKEVYDH ARRALYNAGVVDLVSRNNQS PSHSPLKSSESSMNCSSCEG LSCQQTRVLQEKLRKLKEAM LCMVCCEEEINSTFCPCGHT VCCESCAAQLQSCPVCRSRV EHVQHVYLPTHTSLLNLTVI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 266-445 of human MYLIP (NP_037394.2). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. |
| Post Translational Modifications | Autoubiquitinated. |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. |
| Protein Name | E3 Ubiquitin-Protein Ligase MylipInducible Degrader Of The Ldl-ReceptorIdolMyosin Regulatory Light Chain Interacting ProteinMirRing-Type E3 Ubiquitin Transferase Mylip |
| Database Links | Reactome: R-HSA-8866427Reactome: R-HSA-9031525Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmCell MembranePeripheral Membrane Protein |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Mylip antibodyAnti-Inducible Degrader Of The Ldl-Receptor antibodyAnti-Idol antibodyAnti-Myosin Regulatory Light Chain Interacting Protein antibodyAnti-Mir antibodyAnti-Ring-Type E3 Ubiquitin Transferase Mylip antibodyAnti-MYLIP antibodyAnti-BZF1 antibodyAnti-IDOL antibodyAnti-BM-023 antibodyAnti-PP5242 antibody |
Information sourced from Uniprot.org