| Short Description : | Rabbit polyclonal Myosin Light Chain 6b (1-208) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MYL6B |
| Gene ID: | 140465 |
| Uniprot ID: | MYL6B_HUMAN |
| Immunogen Region: | 1-208 |
| Immunogen Sequence: | MPPKKDVPVKKPAGPSISKP AAKPAAAGAPPAKTKAEPAV PQAPQKTQEPPVDLSKVVIE FNKDQLEEFKEAFELFDRVG DGKILYSQCGDVMRALGQNP TNAEVLKVLGNPKSDELKSR RVDFETFLPMLQAVAKNRGQ GTYEDYLEGFRVFDKEGNGK VMGAELRHVLTTLGEKMTEE EVETVLAGHEDSNGCINYEA FLKHILSV |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-208 of human MYL6B (NP_002466.1). |
| Function | Regulatory light chain of myosin. Does not bind calcium. |
| Protein Name | Myosin Light Chain 6bMyosin Light Chain 1 Slow-Twitch Muscle A IsoformMlc1saSmooth Muscle And Nonmuscle Myosin Light Chain Alkali 6b |
| Database Links | Reactome: R-HSA-445355 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Myosin Light Chain 6b antibodyAnti-Myosin Light Chain 1 Slow-Twitch Muscle A Isoform antibodyAnti-Mlc1sa antibodyAnti-Smooth Muscle And Nonmuscle Myosin Light Chain Alkali 6b antibodyAnti-MYL6B antibodyAnti-MLC1SA antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and MYL6B knockout (KO) 293T cells, using [KO Validated] MYL6B Rabbit polyclonal antibody (STJ11100075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89145/357595/STJ11100075_1__06892.1713121734.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MYL6B knockout (KO) 293T cells, using [KO Validated] MYL6B Rabbit polyclonal antibody (STJ11100075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
